| 质粒名称: | pcdna nesprin TS |
|---|---|
| 出品公司: | addgene |
| 目录编号: | 68127 |
| 存储实验室: | Daniel Conway |
|
载体骨架基本信息 |
|
| 载体名称: | pcdna 3.1 + |
| 载体出品公司: | Invitrogen |
| 空载体大小: | 5428bp |
| 完整质粒大小: | 9476 bp |
| 载体类型: | 哺乳细胞表达 |
| 筛选标记: | 新霉素（G418） |
| 载体抗性: | 氨苄霉素 |
| 生长温度: | 37 ℃ |
| 宿主菌: | DH5a |
| 拷贝数: | 高拷贝 |
|
插入基因信息 |
|
| 插入基因名称: | mouse nesprin mini 2G/SYNE2 |
| 物种来源: | M. musculus (mouse) |
| 插入大小: | 4095 bp |
| 基因突变: | Syne2 (a.k.a. 6820443O06Rik, Cpfl8, D12Ertd777e, KASH2, NUA, Nesp2g, dice, mKIAA1011) |
| 启动子: | CMV |
| 克隆方法: | 限制性内切酶 |
| 5’克隆位点: | HindIII(未破坏) |
| 3’克隆位点: | EcoRI (未破坏) |
| 5’测序引物 | CMV forward |
| 3’测序引物 | BGH rev |
|
文献引用方法 |
|
| 材料和方法部分: | pcdna nesprin TS was a gift from Daniel Conway (Addgene plasmid # 68127 ; http://n2t.net/addgene:68127 ; RRID:Addgene_68127) |
| 参考文献部分: | Nesprin-2G, a Component of the Nuclear LINC Complex, Is Subject to Myosin-Dependent Tension. Arsenovic PT, Ramachandran I, Bathula K, Zhu R, Narang JD, Noll NA, Lemmon CA, Gundersen GG, Conway DE. Biophys J. 2016 Jan 5;110(1):34-43. doi: 10.1016/j.bpj.2015.11.014. 10.1016/j.bpj.2015.11.014 PubMed 26745407 |
买家导航
载体基本信息
载体图谱质粒图谱和多克隆位点信息
载体简介
载体序列
LOCUS pcdna nesprin TS 9476 bp ds-DNA circular SYN 12-MAY-2021
DEFINITION nesprin tension sensor.
ACCESSION .
VERSION .
KEYWORDS pcdna nesprin TS
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 9476)
AUTHORS Arsenovic PT, Ramachandran I, Bathula K, Zhu R, Narang JD, Noll NA,
Lemmon CA, Gundersen GG, Conway DE
TITLE Nesprin-2G, a Component of the Nuclear LINC Complex, Is Subject to
Myosin-Dependent Tension.
JOURNAL Biophys J. 2016 Jan 5;110(1):34-43. doi: 10.1016/j.bpj.2015.11.014.
PUBMED 26745407
REFERENCE 2 (bases 1 to 9476)
AUTHORS .
TITLE Direct Submission
JOURNAL Exported May 12, 2021 from SnapGene Server 1.1.58
http://www.biofeng.com
FEATURES Location/Qualifiers
source 1..9476
/organism="synthetic DNA construct"
/mol_type="other DNA"
primer_bind complement(44..63)
/label=pRS-marker
/note="pRS vectors, use to sequence yeast selectable
marker"
enhancer 235..614
/label=CMV enhancer
/note="human cytomegalovirus immediate early enhancer"
promoter 615..818
/label=CMV promoter
/note="human cytomegalovirus (CMV) immediate early
promoter"
primer_bind 769..789
/label=CMV-F
/note="Human CMV immediate early promoter, forward primer"
primer_bind 863..882
/label=T7
/note="T7 promoter, forward primer"
promoter 863..881
/label=T7 promoter
/note="promoter for bacteriophage T7 RNA polymerase"
regulatory 923..932
/regulatory_class="other"
/note="vertebrate consensus sequence for strong initiation
of translation (Kozak, 1987)"
CDS 2390..3097
/codon_start=1
/product="monomeric teal (cyan) fluorescent protein (Ai et
al., 2006)"
/label=mTFP1
/note="mammalian codon-optimized"
/translation="MVSKGEETTMGVIKPDMKIKLKMEGNVNGHAFVIEGEGEGKPYDG
TNTINLEVKEGAPLPFSYDILTTAFAYGNRAFTKYPDDIPNYFKQSFPEGYSWERTMTF
EDKGIVKVKSDISMEEDSFIYEIHLKGENFPPNGPVMQKKTTGWDASTERMYVRDGVLK
GDVKHKLLLEGGGHHRVDFKTIYRAKKAVKLPDYHFVDHRIEILNHDKDYNKVTVYESA
VARNSTDGMDELYK"
CDS 3218..3934
/codon_start=1
/product="Venus YFP with monomerizing A206K mutation (Nagai
et al., 2002; Kremers et al., 2006)"
/label=mVenus
/note="mammalian codon-optimized"
/translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
KLICTTGKLPVPWPTLVTTLGYGLQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYITADKQKNGIK
ANFKIRHNIEDGGVQLADHYQQNTPIGDGPVLLPDNHYLSYQSKLSKDPNEKRDHMVLL
EFVTAAGITLGMDELYK"
primer_bind complement(3263..3284)
/label=EGFP-N
/note="EGFP, reverse primer"
primer_bind complement(3524..3543)
/label=EXFP-R
/note="For distinguishing EGFP variants, reverse primer"
primer_bind 3871..3892
/label=EGFP-C
/note="EGFP, forward primer"
primer_bind complement(5070..5087)
/label=BGH-rev
/note="Bovine growth hormone terminator, reverse primer.
Also called BGH reverse"
polyA_signal 5076..5300
/label=bGH poly(A) signal
/note="bovine growth hormone polyadenylation signal"
rep_origin 5346..5774
/direction=RIGHT
/label=f1 ori
/note="f1 bacteriophage origin of replication; arrow
indicates direction of (+) strand synthesis"
primer_bind complement(5433..5452)
/label=F1ori-R
/note="F1 origin, reverse primer"
primer_bind 5643..5664
/label=F1ori-F
/note="F1 origin, forward primer"
primer_bind complement(5783..5803)
/label=pBABE 3'
/note="SV40 enhancer, reverse primer for pBABE vectors"
promoter 5788..6117
/label=SV40 promoter
/note="SV40 enhancer and early promoter"
rep_origin 5968..6103
/label=SV40 ori
/note="SV40 origin of replication"
primer_bind 6030..6049
/label=SV40pro-F
/note="SV40 promoter/origin, forward primer"
CDS 6184..6978
/codon_start=1
/gene="aph(3')-II (or nptII)"
/product="aminoglycoside phosphotransferase from Tn5"
/label=NeoR/KanR
/note="confers resistance to neomycin, kanamycin, and G418
(Geneticin(R))"
/translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
primer_bind complement(6238..6257)
/label=Neo-R
/note="Neomycin resistance gene, reverse primer"
primer_bind 6848..6867
/label=Neo-F
/note="Neomycin resistance gene, forward primer"
polyA_signal 7152..7273
/label=SV40 poly(A) signal
/note="SV40 polyadenylation signal"
primer_bind complement(7189..7208)
/label=SV40pA-R
/note="SV40 polyA, reverse primer"
primer_bind 7243..7262
/label=EBV-rev
/note="SV40 polyA terminator, reverse primer"
primer_bind complement(7322..7338)
/label=M13 rev
/note="common sequencing primer, one of multiple similar
variants"
primer_bind complement(7322..7338)
/label=M13 Reverse
/note="In lacZ gene. Also called M13-rev"
primer_bind complement(7335..7357)
/label=M13/pUC Reverse
/note="In lacZ gene"
protein_bind 7346..7362
/label=lac operator
/bound_moiety="lac repressor encoded by lacI"
/note="The lac repressor binds to the lac operator to
inhibit transcription in E. coli. This inhibition can be
relieved by adding lactose or
isopropyl-beta-D-thiogalactopyranoside (IPTG)."
promoter complement(7370..7400)
/label=lac promoter
/note="promoter for the E. coli lac operon"
protein_bind 7415..7436
/label=CAP binding site
/bound_moiety="E. coli catabolite activator protein"
/note="CAP binding activates transcription in the presence
of cAMP."
primer_bind complement(7553..7570)
/label=L4440
/note="L4440 vector, forward primer"
rep_origin complement(7724..8309)
/direction=LEFT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
primer_bind complement(7804..7823)
/label=pBR322ori-F
/note="pBR322 origin, forward primer"
CDS complement(8480..9340)
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/label=AmpR
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
primer_bind 9103..9122
/label=Amp-R
/note="Ampicillin resistance gene, reverse primer"
promoter complement(9341..9445)
/gene="bla"
/label=AmpR promoter
ORIGIN
1 gacggatcgg gagatctccc gatcccctat ggtgcactct cagtacaatc tgctctgatg
61 ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg
121 cgagcaaaat ttaagctaca acaaggcaag gcttgaccga caattgcatg aagaatctgc
181 ttagggttag gcgttttgcg ctgcttcgcg atgtacgggc cagatatacg cgttgacatt
241 gattattgac tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata
301 tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc
361 cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc
421 attgacgtca atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt
481 atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt
541 atgcccagta catgacctta tgggactttc ctacttggca gtacatctac gtattagtca
601 tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg
661 actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc
721 aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg
781 gtaggcgtgt acggtgggag gtctatataa gcagagctct ctggctaact agagaaccca
841 ctgcttactg gcttatcgaa attaatacga ctcactatag ggagacccaa gctggctagc
901 gtttaaactt aagctttcta gagccaccat ggccgctagc cctgtgctgc ccacggaaga
961 tggagagggt ttcttgggca ttgatgatct acacttttca ctgcaagctg agcaggagga
1021 cactcagaag aaaaccttca cctgctggat aaactcacag ctggcaaagc acacacctcc
1081 ctcagttgta tctgacttat tcgcagacat caagaagggg catgtcctcc tggacctgtt
1141 ggaagtcctc tcgggacagc agttgcctcg ggataaagga tctaatacat tccagtgcag
1201 aatcaacata gaacatgccc tgacattcct gaaaaacaga tctatcaagc taataaatat
1261 ccatgttgct gacattgtag aaggaaaccc gtccattatc cttggcctga tctggaccat
1321 catcctgcac tttcatattg agaagctcgc tcagactctg tcttgcgatt acaatcaacc
1381 ttcaccggag gttgtgagcg tggctgcctc atctcctacc tcaagtcctc ccactaagaa
1441 gtgctccaag gctcaagctc aggcaagatg gcagtggtct gccaagaagg ctcttctgca
1501 gtgggctcag gagcagtgtg ccaggtctga gtctgtcaat gtgacagatt ttaagtcaag
1561 ctggagaaat gggatggctt ttttggctgt cattcatgcc ttgcgaccag acctgattga
1621 catggacagc atgaggcaca gatccaacaa agacaatctg aaagaggcct tcaggatcgc
1681 agaacatgag ctgaagatcc ccaaattgct ggaaccagaa gatgtagatg tcgtgaatcc
1741 tgatgagaag tccatcatga cttacgtggc gcagttcctg aagtattcga aggatgcgcc
1801 tggccccggg gattccacac aggccaaggt aagggatgct ctggtctggc taactctgca
1861 agagaagaga ttccagaaga tgctgaagga ctcagcaagt gagacctact gtaacaagta
1921 tcatagtctg ctgtcattta tggagtcact gaatgaagaa aaggaatcct tcattgatgt
1981 cctgtcactg aaagggagga tgggagagct gaatgaagat gaatcacggc tgagacaagg
2041 ctggaccagc ctcatgcacc aggtcgctgc gtggagagcc caactggatg acgccttgcc
2101 ttcaccgctc aaagagaccg aagcgtggct gaaggacata gagggtgttg tgcaagaagg
2161 ggtgccgacc tcccagagtt actctgaagc caggactctg atccaaggga aactatcttc
2221 attcaagagt ctgatgggca gctttgacta tcattcggat gttctcatgg cctttcaatc
2281 caatgccgag aagagcctgc cagctgtgcc tcctgttaaa ctggaggaga tgacaagacg
2341 aatcaacaac gttttgggta aaaacttcat cccactcctg gagctcgaga tggtgagcaa
2401 gggcgaggag accacaatgg gcgtaatcaa gcccgacatg aagatcaagc tgaagatgga
2461 gggcaacgtg aatggccacg ccttcgtgat cgagggcgag ggcgagggca agccctacga
2521 cggcaccaac accatcaacc tggaggtgaa ggagggagcc cccctgccct tctcctacga
2581 cattctgacc accgcgttcg cctacggcaa cagggccttc accaagtacc ccgacgacat
2641 ccccaactac ttcaagcagt ccttccccga gggctactct tgggagcgca ccatgacctt
2701 cgaggacaag ggcatcgtga aggtgaagtc cgacatctcc atggaggagg actccttcat
2761 ctacgagata cacctcaagg gcgagaactt cccccccaac ggccccgtga tgcagaagaa
2821 gaccaccggc tgggacgcct ccaccgagag gatgtacgtg cgcgacggcg tgctgaaggg
2881 cgacgtcaag cacaagctgc tgctggaggg cggcggccac caccgcgttg acttcaagac
2941 catctacagg gccaagaagg cggtgaagct gcccgactat cactttgtgg accaccgcat
3001 cgagatcctg aaccacgaca aggactacaa caaggtgacc gtttacgaga gcgccgtggc
3061 ccgcaactcc accgacggca tggacgagct gtacaagggg ccaggtggtg cagggccagg
3121 tggtgcaggg ccaggtggtg cagggccagg tggtgcaggg cccggtggtg caggtccagg
3181 tggtgcaggt ccaggtggtg caggtccagg tggtgctatg gtgagcaagg gcgaggagct
3241 gttcaccggg gtggtgccca tcctggtcga gctggacggc gacgtaaacg gccacaagtt
3301 cagcgtgtcc ggcgagggcg agggcgatgc cacctacggc aagctgaccc tgaagctgat
3361 ctgcaccacc ggcaagctgc ccgtgccctg gcccaccctc gtgaccaccc tgggctacgg
3421 cctgcagtgc ttcgcccgct accccgacca catgaagcag cacgacttct tcaagtccgc
3481 catgcccgaa ggctacgtcc aggagcgcac catcttcttc aaggacgacg gcaactacaa
3541 gacccgcgcc gaggtgaagt tcgagggcga caccctggtg aaccgcatcg agctgaaggg
3601 catcgacttc aaggaggacg gcaacatcct ggggcacaag ctggagtaca actacaacag
3661 ccacaacgtc tatatcaccg ccgacaagca gaagaacggc atcaaggcca acttcaagat
3721 ccgccacaac atcgaggacg gcggcgtgca gctcgccgac cactaccagc agaacacccc
3781 catcggcgac ggccccgtgc tgctgcccga caaccactac ctgagctacc agtccaagct
3841 gagcaaagac cccaacgaga agcgcgatca catggtcctg ctggagttcg tgaccgccgc
3901 cgggatcact ctcggcatgg acgagctgta caaggcggcc gcgcaggagc ttcacagcaa
3961 gctcaggctc aaacagaccg tgcagcagct caagtctgac atcggcagca tcgctgcttg
4021 gcttgggaaa actgaggccg agctggaagc tctgaagttg gccgagcctc cctctgacat
4081 ccaggaaata gcgctcaggg tgaagagact gcaagagata ctaaaagcct tcgataccta
4141 caaggccttg atggtctccg tcaatgtgag ccacaaggaa tatctgccaa gccagagccc
4201 cgaagccaca gaactccaaa atagactcca tcagctgagc ctgtcctggg actcggtcca
4261 gggcgtattg gacagctggc gaggagactt acggcagtca ctcatgcagt gccaggactt
4321 tcaccagctg agtcaagact tgctcctgtg gctagcgaca gcagagagcc gcaggcagaa
4381 ggcgcatgtc accagcccag aggccgaccg ccaggtcctc ctggagtgtc agaaagacct
4441 aatgcgccta gaaaaggagt tggtagcccg tcagcctcaa gtcagctcct tgcgggagat
4501 ttcctccagc cttctggtga aggggcaagg agaagactac attgaggccg aagagaaggt
4561 ccacgtgatc gagaagaaac tcaagcaact gcaagagcaa gtggctcaag atctgatgtc
4621 cttgcagaga agcctggatc cggatgcttc tctaaccagc tttgatgagg tggactcagg
4681 agaacagctc ccagcagcct ttgcaaagag tccaaggccc cgctggacct tcttggaaga
4741 ggaagaggag gaggaggaga cagacagcag gatgccccac ctcgacagcc ccggcagctc
4801 ccagccgaga cgctccttcc tctcaagggt gatcagggca gcgctaccgt tgcagctgct
4861 tctgctgctg ctgctgctcc tggcctgcct gctacctgcc tctgaagatg actacagctg
4921 cacccaggcc aacaactttg cccgatcctt ctaccccatg ctgcggtaca ccaacgggcc
4981 acctcccacc tagaccggtg aattctgcag atatccagca cagtggcggc cgctcgagtc
5041 tagagggccc gtttaaaccc gctgatcagc ctcgactgtg ccttctagtt gccagccatc
5101 tgttgtttgc ccctcccccg tgccttcctt gaccctggaa ggtgccactc ccactgtcct
5161 ttcctaataa aatgaggaaa ttgcatcgca ttgtctgagt aggtgtcatt ctattctggg
5221 gggtggggtg gggcaggaca gcaaggggga ggattgggaa gacaatagca ggcatgctgg
5281 ggatgcggtg ggctctatgg cttctgaggc ggaaagaacc agctggggct ctagggggta
5341 tccccacgcg ccctgtagcg gcgcattaag cgcggcgggt gtggtggtta cgcgcagcgt
5401 gaccgctaca cttgccagcg ccctagcgcc cgctcctttc gctttcttcc cttcctttct
5461 cgccacgttc gccggctttc cccgtcaagc tctaaatcgg gggctccctt tagggttccg
5521 atttagtgct ttacggcacc tcgaccccaa aaaacttgat tagggtgatg gttcacgtag
5581 tgggccatcg ccctgataga cggtttttcg ccctttgacg ttggagtcca cgttctttaa
5641 tagtggactc ttgttccaaa ctggaacaac actcaaccct atctcggtct attcttttga
5701 tttataaggg attttgccga tttcggccta ttggttaaaa aatgagctga tttaacaaaa
5761 atttaacgcg aattaattct gtggaatgtg tgtcagttag ggtgtggaaa gtccccaggc
5821 tccccagcag gcagaagtat gcaaagcatg catctcaatt agtcagcaac caggtgtgga
5881 aagtccccag gctccccagc aggcagaagt atgcaaagca tgcatctcaa ttagtcagca
5941 accatagtcc cgcccctaac tccgcccatc ccgcccctaa ctccgcccag ttccgcccat
6001 tctccgcccc atggctgact aatttttttt atttatgcag aggccgaggc cgcctctgcc
6061 tctgagctat tccagaagta gtgaggaggc ttttttggag gcctaggctt ttgcaaaaag
6121 ctcccgggag cttgtatatc cattttcgga tctgatcaag agacaggatg aggatcgttt
6181 cgcatgattg aacaagatgg attgcacgca ggttctccgg ccgcttgggt ggagaggcta
6241 ttcggctatg actgggcaca acagacaatc ggctgctctg atgccgccgt gttccggctg
6301 tcagcgcagg ggcgcccggt tctttttgtc aagaccgacc tgtccggtgc cctgaatgaa
6361 ctgcaggacg aggcagcgcg gctatcgtgg ctggccacga cgggcgttcc ttgcgcagct
6421 gtgctcgacg ttgtcactga agcgggaagg gactggctgc tattgggcga agtgccgggg
6481 caggatctcc tgtcatctca ccttgctcct gccgagaaag tatccatcat ggctgatgca
6541 atgcggcggc tgcatacgct tgatccggct acctgcccat tcgaccacca agcgaaacat
6601 cgcatcgagc gagcacgtac tcggatggaa gccggtcttg tcgatcagga tgatctggac
6661 gaagagcatc aggggctcgc gccagccgaa ctgttcgcca ggctcaaggc gcgcatgccc
6721 gacggcgagg atctcgtcgt gacccatggc gatgcctgct tgccgaatat catggtggaa
6781 aatggccgct tttctggatt catcgactgt ggccggctgg gtgtggcgga ccgctatcag
6841 gacatagcgt tggctacccg tgatattgct gaagagcttg gcggcgaatg ggctgaccgc
6901 ttcctcgtgc tttacggtat cgccgctccc gattcgcagc gcatcgcctt ctatcgcctt
6961 cttgacgagt tcttctgagc gggactctgg ggttcgaaat gaccgaccaa gcgacgccca
7021 acctgccatc acgagatttc gattccaccg ccgccttcta tgaaaggttg ggcttcggaa
7081 tcgttttccg ggacgccggc tggatgatcc tccagcgcgg ggatctcatg ctggagttct
7141 tcgcccaccc caacttgttt attgcagctt ataatggtta caaataaagc aatagcatca
7201 caaatttcac aaataaagca tttttttcac tgcattctag ttgtggtttg tccaaactca
7261 tcaatgtatc ttatcatgtc tgtataccgt cgacctctag ctagagcttg gcgtaatcat
7321 ggtcatagct gtttcctgtg tgaaattgtt atccgctcac aattccacac aacatacgag
7381 ccggaagcat aaagtgtaaa gcctggggtg cctaatgagt gagctaactc acattaattg
7441 cgttgcgctc actgcccgct ttccagtcgg gaaacctgtc gtgccagctg cattaatgaa
7501 tcggccaacg cgcggggaga ggcggtttgc gtattgggcg ctcttccgct tcctcgctca
7561 ctgactcgct gcgctcggtc gttcggctgc ggcgagcggt atcagctcac tcaaaggcgg
7621 taatacggtt atccacagaa tcaggggata acgcaggaaa gaacatgtga gcaaaaggcc
7681 agcaaaaggc caggaaccgt aaaaaggccg cgttgctggc gtttttccat aggctccgcc
7741 cccctgacga gcatcacaaa aatcgacgct caagtcagag gtggcgaaac ccgacaggac
7801 tataaagata ccaggcgttt ccccctggaa gctccctcgt gcgctctcct gttccgaccc
7861 tgccgcttac cggatacctg tccgcctttc tcccttcggg aagcgtggcg ctttctcata
7921 gctcacgctg taggtatctc agttcggtgt aggtcgttcg ctccaagctg ggctgtgtgc
7981 acgaaccccc cgttcagccc gaccgctgcg ccttatccgg taactatcgt cttgagtcca
8041 acccggtaag acacgactta tcgccactgg cagcagccac tggtaacagg attagcagag
8101 cgaggtatgt aggcggtgct acagagttct tgaagtggtg gcctaactac ggctacacta
8161 gaagaacagt atttggtatc tgcgctctgc tgaagccagt taccttcgga aaaagagttg
8221 gtagctcttg atccggcaaa caaaccaccg ctggtagcgg tttttttgtt tgcaagcagc
8281 agattacgcg cagaaaaaaa ggatctcaag aagatccttt gatcttttct acggggtctg
8341 acgctcagtg gaacgaaaac tcacgttaag ggattttggt catgagatta tcaaaaagga
8401 tcttcaccta gatcctttta aattaaaaat gaagttttaa atcaatctaa agtatatatg
8461 agtaaacttg gtctgacagt taccaatgct taatcagtga ggcacctatc tcagcgatct
8521 gtctatttcg ttcatccata gttgcctgac tccccgtcgt gtagataact acgatacggg
8581 agggcttacc atctggcccc agtgctgcaa tgataccgcg agacccacgc tcaccggctc
8641 cagatttatc agcaataaac cagccagccg gaagggccga gcgcagaagt ggtcctgcaa
8701 ctttatccgc ctccatccag tctattaatt gttgccggga agctagagta agtagttcgc
8761 cagttaatag tttgcgcaac gttgttgcca ttgctacagg catcgtggtg tcacgctcgt
8821 cgtttggtat ggcttcattc agctccggtt cccaacgatc aaggcgagtt acatgatccc
8881 ccatgttgtg caaaaaagcg gttagctcct tcggtcctcc gatcgttgtc agaagtaagt
8941 tggccgcagt gttatcactc atggttatgg cagcactgca taattctctt actgtcatgc
9001 catccgtaag atgcttttct gtgactggtg agtactcaac caagtcattc tgagaatagt
9061 gtatgcggcg accgagttgc tcttgcccgg cgtcaatacg ggataatacc gcgccacata
9121 gcagaacttt aaaagtgctc atcattggaa aacgttcttc ggggcgaaaa ctctcaagga
9181 tcttaccgct gttgagatcc agttcgatgt aacccactcg tgcacccaac tgatcttcag
9241 catcttttac tttcaccagc gtttctgggt gagcaaaaac aggaaggcaa aatgccgcaa
9301 aaaagggaat aagggcgaca cggaaatgtt gaatactcat actcttcctt tttcaatatt
9361 attgaagcat ttatcagggt tattgtctca tgagcggata catatttgaa tgtatttaga
9421 aaaataaaca aataggggtt ccgcgcacat ttccccgaaa agtgccacct gacgtc
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